1. Nonacus Support
  2. Cell3™ Target
  3. Analysing Cell3™ Target sequencing data

Do I set up my sequencer differently for applications with or without UMIs?

No, you set up the sequencer the same for all applications. The 9bp UMI is attached to the Illumina i7 8bp index and therefore, needs 17 cycles. The i5 index has 8bp and needs 8 cycles. Then you can choose to use them or not during your bioinformatics analysis according to the application.