1. Nonacus Support
  2. Cell3™ Target
  3. Cell3™ Target Library preparation

I am preparing to do whole genome-seq library prep on FFPE DNA with low DIN values but high concentration. How much starting material would you advise I use for the library preps?

Are there any parts of the protocol I would need to change for a whole genome-seq library prep (vs exome-seq), such as fragmentation time?

As you have plenty of DNA for your WGS, and therefore not using the UMIs, we recommend performing a PCR free method to eliminate PCR bias. To do this you would need a minimum of 100ng of DNA to input and if your FFPE DNA has very low DIN scores (e.g. less than 3) you would need to increase your DNA input by 5-10 fold (see page 11 in Cell3 Target protocol).