1. Nonacus Support
  2. Cell3™ Target
  3. Cell3™ Target Library preparation: methods, equipment and reagents

I am using Cell3™ Xtract for cfDNA extraction from plasma or serum.

I notice that your elution buffer contains EDTA, which is incompatible with my downstream protocols. Can I elute in water instead of the supplied buffer?

Our elution buffer consists of 10 mM Tris-HCl, pH 8.5, 0.1 mM EDTA; therefore, this low level of EDTA should not affect most downstream applications, including NGS library prep. It  is perfectly fine to use water in place of the supplied buffer to elute your cfDNA. If doing so, please ensure that the pH is >6.0. 

However, if you are storing your samples the buffer should be used. In addition, it is advisable to only extract cfDNA at the point where you plan to proceed with the library preparation.