Cell3™ Direct qPCR technology is extremely sensitive, as few as 1-3 copies can be amplified. Amplification of the NTCs or negative controls is likely to be due to low level contamination.
We recommend the following actions:
- Use clean working practises to minimise the potential of template contamination.
- Where possible use separate areas for PCR mix preparation, template addition and qPCR reaction running.
- Set up qPCR reaction in a PCR or biological safety cabinet using sterile / PCR-clean equipment and consumables only.