Why are my Non Template Controls (NTCs) or negative controls amplifying?

Cell3™ Direct qPCR technology is extremely sensitive, as few as 1-3 copies can be amplified. Amplification of the NTCs or negative controls is likely to be due to low level contamination.

We recommend the following actions:

  • Use clean working practises to minimise the potential of template contamination.
  • Where possible use separate areas for PCR mix preparation, template addition and qPCR reaction running.
  • Set up qPCR reaction in a PCR or biological safety cabinet using sterile / PCR-clean equipment and consumables only.