Cell3™ Target Library preparation: methods, equipment and reagents
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      1. Nonacus Support
      2. Cell3™ Target
      3. Cell3™ Target Library preparation: methods, equipment and reagents

      The maximum temperature for the lids of our cyclers is 99˚C. Is it possible to reduce temperature of the denaturation steps in step 1D (library amplification) to 95˚C with a lid heated to 99˚C?

      A heated lid that has a max of 99˚C should work fine. However, we would not recommend adjusting the denaturation temperature to 95 ˚C in the library amplification step and recommend keeping this at 98 ˚C.