1. Nonacus Support
  2. Cell3™ Target
  3. Cell3™ Target Library preparation: methods, equipment and reagents

The maximum temperature for the lids of our cyclers is 99˚C. Is it possible to reduce temperature of the denaturation steps in step 1D (library amplification) to 95˚C with a lid heated to 99˚C?

A heated lid that has a max of 99˚C should work fine. However, we would not recommend adjusting the denaturation temperature to 95 ˚C in the library amplification step and recommend keeping this at 98 ˚C.