1. Nonacus Support
  2. Cell3™ Target
  3. Sequencing Cell3™ Target libraries

What are the input DNA requirements for FFPE samples ?

DNA extracted from formalin-fixed paraffin-embedded (FFPE) tissue is generally more degraded than genomic DNA extracted from fresh tissue or cells and can be chemically modified to different degrees. Depending on the level of DNA degradation, increasing quantities of input DNA need to be used during library preparation in order to achieve similar yields compared to high-purity DNA. The DNA integrity score (or DIN score) can be determined by running FFPE DNA samples on an Agilent Genomic DNA ScreenTape (Agilent Technologies). The following table provides a guideline on FFPE DNA input quantities to use according to the DIN score observed: 

_Cell3 Target updated protocol branding.indd @ 98% 07_02_2022 15_43_12 (2)